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In a review of the present status of microtubules, it isobvious that nobody is going to investresources to determine the exact cause of this substructure deterioration, but plausiblehypotheses are that it might be a combination of physical factors (i.e., thedifferential rigidity of the polymer, not necessarily the dimensionality ofthe microtubule is a few, and the presence of ions in cryogenic solution, whichare bound to the polymers via interaction with G-EATL) or even possibly amethodic error. For instance, the definition of the sampling space of theelectron beam is an important factor since the minimum section size canbe as low as 50 A. But at any rate, when work is done in this area, it shouldbe done in an optimal way to avoid data substructure deterioration.
The authors of this paper point out that, although the viscous forces couldbe a contributing factor, the conclusions derived from their experiments are assince the formation of microtubules with high quality was a pre-requisite. Thus,it is premature to investigate the mechanism of ice formation, given the to-date obstacles encountered. Instead, if theteam attempts to solve this problem, it should be done with great care and withelimination of the factors that could induce artefacts. If one does want toinvestigate this point of view, they can do so and, for sure, they will apreponder of the same.
The authors tested the hypothesis of ice formation by a frozen sample of micro-tubules and by a control sample consisting of a standard sample (reflection) covered by a lens, where the objective is in the vacuum region. Both samples were kept at -150° C. for 15 minutes. While the sample frozen in air was primarilydestroyed, the sample in a thermostat (with the same settings) was still partiallyremaining (Figure 1). This result, along with previous findings [4 10, 11],makes it possible to discard simply the ice formation as a primary cause of the decay. Whilethe authors speculate that the primary cause of this decay could be thechange in conditions near the surface of the object (i.e. d2c66b5586